The following sequence of topics is recommended for Grade 12 based on the progressive development of concepts through the different topics:

1. DNA: The Code of Life

2. Meiosis

3. Reproduction in Vertebrates

4. Human Reproduction

5. Genetics and Inheritance

6. Responding to the Environment (Humans)

7. Human Endocrine System

8. Homeostasis in Humans

9. Responding to the Environment (Plants)

10. Evolution

11. Human Impact (from Grade 11)





DNA: location, structure and functions
Location of DNA:

• Makes up the genes on chromosomes (nuclear DNA)

• Present in mitochondria (mitochondrial DNA)

Brief history of the discovery of the DNA molecule (Watson & Crick, Franklin & Wilkins)

Three components of a DNA nucleotide:

• Nitrogenous bases linked by weak hydrogen bonds:

- Four nitrogenous bases of DNA: adenine (A), thymine (T), cytosine (C), guanine (G)

- Pairing of bases in DNA occur as follows: A : T and G : C

• Sugar portion (deoxyribose in DNA)

• Phosphate portion

The natural shape of the DNA molecule is a double helix

Stick diagram of DNA molecule to illustrate its structure

Functions of DNA:

• Sections of DNA-forming genes carry hereditary information

• DNA contains coded information for protein synthesis

DNA replication
Process of DNA replication:

• When in the cell cycle it takes place

• Where in the cell it takes place

• How DNA replication takes place (names of enzymes not required)

• The significance of DNA replication

DNA profiling

Definition of DNA profile

Uses of DNA profiles

Interpretation of DNA profiles

RNA: location, structure and function
Location of RNA:

• mRNA is formed in the nucleus and functions on the ribosome

• tRNA is located in the cytoplasm

RNA plays a role in protein synthesis

Structure of RNA:

A single-stranded molecule consisting of nucleotides

Each nucleotide is made up of a sugar (ribose), phosphate and a nitrogen base

Four nitrogenous bases of RNA: adenine (A), uracil (U), cytosine (C), guanine (G)

Stick diagram of mRNA and tRNA molecules to illustrate their structure

Protein synthesis
The involvement of DNA and RNA in protein synthesis:

• Transcription

- The double helix DNA unwinds.

- The double-stranded DNA unzips/weak hydrogen bonds break

- to form two separate strands.

- One strand is used as a template

- to form mRNA

- using free RNA nucleotides from the nucleoplasm.

- The mRNA is complementary to the DNA.

- mRNA now has the coded message for protein synthesis.

• mRNA moves from the nucleus to the cytoplasm and attaches to the ribosome.

• Translation

- Each tRNA carries a specific amino acid.

- When the anticodon on the tRNA

- matches the codon on the mRNA

- then tRNA brings the required amino acid to the ribosome. (Names of specific codons, anticodons and their amino acids are not to be memorised.)

- Amino acids become attached by peptide bonds

- to form the required protein.

Simple diagram to illustrate transcription and translation in protein synthesis



Introduction

Revision of the structure of a cell with an emphasis on the parts of the nucleus, the centrosome and the cytoplasm

Structure of chromosomes:

• Chromosomes consist of DNA (which makes up genes) and protein

• The number of chromosomes in a cell is a characteristic of an organism (e.g. humans have 46 chromosomes)

• Chromosomes which are single threads become double (two chromatids joined by a centromere) as a result of DNA replication

Differentiate between:

• Haploid (n) and diploid (2n) cells in terms of chromosome number

• Sex cells (gametes) and somatic cells (body cells)

• Sex chromosomes (gonosomes) and autosomes

Revision of the process of mitosis

Meiosis – The process

Definition of meiosis

Site of meiosis in plants and in animals

Meiosis is a continuous process, but the events are divided into different phases for convenience

Events of interphase:

• DNA replication takes place

• Chromosomes which are single threads, become double

• Each chromosome will now consist of two chromatids joined by a centromere

• DNA replication helps to double the genetic material so that it can be shared by the new cells arising from cell division

The events of the following phases of Meiosis I, using diagrams:

• Prophase I

- Including a description of crossing over

• Metaphase I

- Including the random arrangement of chromosomes

• Anaphase I

• Telophase I

The events of each phase of Meiosis II, using diagrams:

• Prophase II

• Metaphase II

- Including the random arrangement of chromosomes

• Anaphase II

• Telophase II

Importance of meiosis

The importance of meiosis:

• Production of haploid gametes

• The halving effect of meiosis overcomes the doubling effect of fertilisation, thus maintaining a constant chromosome number from one generation to the next

• Mechanism to introduce genetic variation through:

- Crossing over

- The random arrangement of chromosomes at the equator

Abnormal meiosis

Non-disjunction and its consequences

Non-disjunction of chromosome pair 21 during Anaphase I in humans to form abnormal gametes with an extra copy of chromosome 21

The fusion between an abnormal gamete (24 chromosomes) and a normal gamete (23 chromosomes) may lead to Down syndrome

Comparison of mitosis and meiosis

Similarities of mitosis and meiosis

Differences between mitosis and meiosis



Diversity of reproductive strategies

The role of the following reproductive strategies in animals in maximising reproductive success in different environments (using relevant examples):

• External fertilisation and internal fertilisation

• Ovipary, ovovivipary and vivipary

• Amniotic egg

• Precocial and altricial development

• Parental care



Introduction

Revision of the schematic outline of the human life cycle to show the role of meiosis, mitosis and fertilisation Structure of the male reproductive system

Structure of the male reproductive system, using a diagram

Functions of the testis, epididymis, vas deferens, seminal vesicle, prostate gland, Cowper's gland and the urethra

Structure of the female reproductive system

Structure of the female reproductive system, using a diagram

Functions of the ovary, Fallopian tubes, uterus lined by endometrium, cervix, vagina with its external opening and the vulva

Structure of the ovary, using a diagram, showing the primary follicles, the Graafian follicle and the corpus luteum

Puberty

Main changes that occur in male characteristics during puberty under the influence of testosterone

Main changes that occur in female characteristics during puberty under the influence of oestrogen

Gametogenesis

Formation of gametes (gametogenesis) by meiosis

• Male gametes formed by spermatogenesis

• Female gametes formed by oogenesis Spermatogenesis:

• Under the influence of testosterone

• diploid cells in the seminiferous tubules of the testes undergo meiosis

• to form haploid sperm cells

Structure of a sperm, using a diagram

Functions of the parts of a sperm cell (acrosome, head with haploid nucleus, middle portion/neck with mitochondria and a tail) Oogenesis:

• Under the influence of FSH

• diploid cells in the ovary undergo mitosis

• to form numerous follicles.

• One cell inside a follicle enlarges and undergoes meiosis.

• Of the four cells that are produced, only one survives to form a mature, haploid ovum.

Structure of an ovum, using a diagram

Functions of the different parts of an ovum (layer of jelly, haploid nucleus, cytoplasm)

Menstrual cycle

The menstrual cycle includes the uterine and ovarian cycles

Events in the ovarian cycle:

• Development of the Graafian follicle

• Ovulation

• Formation of the corpus luteum

Events in the uterine cycle:

• Changes that take place in the thickness of the endometrium

• Menstruation

Hormonal control of the menstrual cycle (ovarian and uterine cycles) with reference to the action of FSH, oestrogen, LH and progesterone

Negative feedback mechanism involving FSH and progesterone in controlling the production of ova

Fertilisation and development of zygote to blastocyst

Definition of copulation and fertilisation

Process of fertilisation

Development of zygote embryo (morula and blastula/blastocyst)

foetus

Implantation, gestation and the role of the placenta

Definition of implantation

The role of oestrogen and progesterone in maintaining pregnancy

Structure of the developing foetus in the uterus, using a diagram

Functions of the following parts:

• Chorion and chorionic villi

• Amnion, amniotic cavity and amniotic fluid

• Umbilical cord (including umbilical artery and umbilical vein)

• Placenta



Introduction

Mention of Mendel as the father of genetics

Concepts in inheritance

Chromatin and chromosomes

Genes and alleles

Dominant and recessive alleles – The Law of Dominance

Phenotype and genotype

Homozygous and heterozygous

Monohybrid crosses

Format for representing a genetics cross

Mendel's Principle of Segregation

Types of dominance:

• Complete dominance – one allele is dominant and the other is recessive, such that the effect of the recessive allele is masked by the dominant allele in the heterozygous condition

• Incomplete dominance – none of the two alleles of a gene is dominant over the other, resulting in an intermediate phenotype in the heterozygous condition

• Co-dominance – both alleles of a gene are equally dominant whereby both alleles express themselves in the phenotype in the heterozygous condition

Genetics problems involving each of the three types of dominance

Proportion and ratio of genotypes and phenotypes

Sex determination

22 pairs of chromosomes in humans are autosomes and one pair of chromosomes are sex chromosomes/gonosomes

Males have XY chromosomes and females have XX chromosomes

Differentiate between sex chromosomes (gonosomes) and autosomes in the karyotypes of human males and females

Representation of a genetic cross to show the inheritance of sex

Sex-linked inheritance

Sex-linked alleles and sex-linked disorders

Genetics problems involving the following sex-linked disorders:

• Haemophilia

• Colour-blindness

Blood grouping

Different blood groups are a result of multiple alleles

The alleles |A,|B and i in different combinations result in four blood groups

Genetics problems involving the inheritance of blood type

Dihybrid crosses

Mendel's Principle of Independent Assortment

Dihybrid genetics problems

Determination of the proportion/ratio of genotypes and phenotypes

Genetic lineages/pedigrees

A genetic lineage/pedigree traces the inheritance of characteristics over many generations

Interpretation of pedigree diagrams Mutations

Definition of a mutation

Effects of mutations: harmful mutations, harmless mutations and useful mutations

Mutations contribute to genetic variation

Definition of gene mutation and chromosomal mutation

Mutations lead to altered characteristics in each of the following genetic disorders:

• Haemophilia – absence of blood-clotting factors

• Colour-blindness – due to absence of the proteins that comprise either the red or green cones/photoreceptors in the eye

• Down syndrome – due to an extra copy of chromosome 21 as a result of non-disjunction during meiosis

Genetic engineering

Genetic engineering uses biotechnology to satisfy human needs:

• Stem cell research – sources and uses of stem cells

• Genetically modified organisms – brief outline of process (names of enzymes involved are not required) and benefits of genetic modification

• Cloning – brief outline of process and benefits of cloning Paternity testing

The role of each of the following in paternity testing:

• Blood grouping

• DNA profiles Genetic links

Mutations in mitochondrial DNA used in tracing female ancestry



Introduction

The nervous system (involving nerves) and endocrine system (involving hormones) are two components that help us respond to the environment

Human nervous system

The need for a nervous system in humans:

• Reaction to stimuli (stimuli can be external and internal)

• Coordination of the various activities of the body

Central nervous system

The brain and spinal cord are protected by meninges

Location and functions of the following parts:

• Brain

- Cerebrum

- Cerebellum

- Corpus callosum

- Medulla oblongata

• Spinal cord

Peripheral nervous system

Location and functions of the peripheral nervous system (cranial and spinal nerves)

Autonomic nervous system

Location and functions of the autonomic nervous system (sympathetic and parasympathetic sections)

Structure and functioning of a nerve

Functions of sensory and motor neurons

Structure and functions of parts of sensory and motor neurons, using diagrams: nucleus, cell body, cytoplasm, myelin sheath, axon and dendrites The simple reflex arc  Definition of reflex action and a reflex arc

Structure of a reflex arc and functions of each part, using a diagram: receptor, sensory neuron, dorsal root of spinal nerve, spinal cord, interneuron, motor neuron, ventral root of spinal nerve, effector

Functioning of a simple reflex action, using an example

Significance of a reflex action

Significance of synapses

Disorders of the CNS Causes and symptoms of the following disorders of the nervous system:

• Alzheimer's disease

• Multiple sclerosis

Receptors

Functions of receptors, neurons and effectors in responding to the environment  The body responds to a variety of different stimuli, such as light, sound, touch, temperature, pressure, pain and chemicals (taste and smell). (No structure and names necessary except for names of the receptors in the eye and ear.) Human eye

Structure and functions of the parts of the human eye, using a diagram

Binocular vision and its importance

The changes that occur in the human eye for each of the following, using

diagrams:

• Accommodation

• Pupillary mechanism

The nature and treatment of the following visual defects, using diagrams:

• Short-sightedness

• Long-sightedness

• Astigmatism

• Cataracts

Human ear

Structure of the human ear and the functions of the different parts, using a diagram

Functioning of the human ear in:

• Hearing (include the role of the organ of Corti, without details of its structure)

• Balance (include the role of maculae and cristae, without details of their structure)

Cause and treatment of the following hearing defects:

• Middle ear infection (the use of grommets)

• Deafness (the use of hearing aids and cochlear implants)



Introduction

Difference between an endocrine and an exocrine gland

Definition of a hormone

Location of each of the following glands, using a diagram, the hormones they secrete and function(s) of each hormone:

• Hypothalamus (ADH)

• Pituitary/Hypophysis (GH, TSH, FSH, LH, prolactin)

• Thyroid glands (thyroxin)

• Islets of Langerhans in the pancreas (insulin, glucagon)

• Adrenal glands (adrenalin, aldosterone)

• Ovary (oestrogen, progesterone)

• Testis (testosterone

Negative feedback mechanism involving:

• TSH and thyroxin (and the result of an imbalance: thyroid disorders)

• Insulin and glucagon (and the result of an imbalance: diabetes mellitus)



Introduction

Homeostasis as the process of maintaining a constant, internal environment within narrow limits, despite changes that take place internally and externally

The conditions within cells depend on the conditions within the internal environment (the tissue fluid)

Factors such as carbon dioxide, glucose, salt and water concentration, temperature and pH must be kept constant in the internal environment (tissue fluid)

Homeostasis through negative feedback

Negative feedback mechanism controlling the concentration of:

• Glucose

• Carbon dioxide

• Water

• Salts

Thermoregulation

Structure of the skin, using a diagram, with an emphasis on the parts involved in thermoregulation

Role of the following in negative feedback mechanism for controlling

temperature/thermoregulation:

• Sweating

• Vasodilation

• Vasoconstriction



Plant hormones

General functions of the following:

• Auxins

• Gibberellins

• Abscisic acid

The control of weeds using plant hormones

The role of auxins in:

• Geotropism

• Phototropism Plant defence mechanisms

Role of the following as plant defence mechanisms:

Chemicals

Thorns



Introduction

Definition of biological evolution

Difference between a hypothesis and a theory

The Theory of Evolution is regarded as a scientific theory since various hypotheses relating to evolution have been tested and verified over time Evidence for evolution

Role of the following as evidence for evolution:

• Fossil record – Link to Grade 10

• Biogeography – Link to Grade 10

• Modification by descent (homologous structures)

• Genetics Variation

Definition of a biological species and a population

A review of the contribution of each of the following to variation that exists amongst individuals of the same species:

• Meiosis

- Crossing over

- Random arrangement of chromosomes

• Mutations

• Random fertilisation

• Random mating

Continuous and discontinuous variation Origin of an idea about origins (a historical development)

Ideas on evolution in the order of their origin are as follows:

• Lamarckism

• Darwinism

• Punctuated Equilibrium Lamarckism (Jean Baptiste de Lamarck – 1744–1829)

Lamarck used two 'laws' to explain evolution:

• 'Law' of use and disuse

• 'Law' of the inheritance of acquired characteristics

Reasons for Lamarck's theory being rejected Darwinism (Charles Darwin – 1809–1882)

Darwin's theory of evolution by natural selection:

• Organisms produce a large number of offspring.

• There is a great deal of variation amongst the offspring.

• Some have favourable characteristics and some do not.

• When there is a change in the environmental conditions or if there is competition,

• then organisms with characteristics, which make them more suited, survive

• whilst organisms with unfavourable characteristics, which make them less suited, die.

• The organisms that survive, reproduce

• and thus pass on the allele for the favourable characteristic to their offspring.

• The next generation will therefore have a higher proportion of individuals with the favourable characteristic.

• In this way, the characteristics of a population gradually change over a long period of time. Punctuated Equilibrium (Eldredge and Gould – 1972)

Punctuated Equilibrium explains the speed at which evolution takes place:

• Evolution involves long periods of time where species do not change or change gradually through natural selection (known as equilibrium).

• This alternates with (is punctuated by) short periods of time where rapid changes occur through natural selection

• during which new species may form in a short period of time. Artificial selection  Artificial selection involving:

• A domesticated animal species

• A crop species Formation of new species

Biological species concept: similar organisms that are capable of interbreeding to produce fertile offspring

Speciation and extinction and the effect of each on biodiversity

Speciation through geographic isolation:

• If a population of a single species

• becomes separated by a geographical barrier (sea, river, mountain, lake)

• then the population splits into two.

• There is now no gene flow between the two populations.

• Since each population may be exposed to different environmental conditions/the selection pressure may be different

• natural selection occurs independently in each of the two populations

• such that the individuals of the two populations become very different from each other

• genotypically and phenotypically.

• Even if the two populations were to mix again

• they will not be able to interbreed.

• The two populations are now different species.

Speciation through geographic isolation in ONE of the following:

• Galapagos finches

• Galapagos tortoises

• Plants on different land masses (linked to continental drift)

- Baobabs in Africa and Madagascar

- Proteas in South Africa and Australia

• Any example of mammals on different land masses Mechanisms of reproductive isolation (Keeping species separate)

A brief outline of reproductive isolation mechanisms that help to keep species separate:

• Breeding at different times of the year

• Species-specific courtship behaviour

• Adaptation to different pollinators

• Infertile offspring

• Prevention of fertilisation

Evolution in present times

Any ONE example of natural selection and evolution in present times:

• Use of insecticides and consequent resistance to insecticides in insects

• Development of resistant strains of tuberculosis-causing bacteria (MDR and XDR) to antibiotics, due to mutations (variations) in bacteria and failure to complete antibiotic courses

• HIV resistance to antiretroviral medication

• Bill (beak) and body size of Galapagos finches Evidence of common ancestors for living hominids, including humans

Interpretation of a phylogenetic tree to show the place of the family Hominidae in the animal kingdom

Characteristics that humans share with African apes

Anatomical differences between African apes and humans, with the aid of diagrams, as it applies to the following characteristics:

• Bipedalism (foramen magnum, spine and pelvic girdle)

• Brain size

• Teeth (dentition)

• Prognathism

• Palate shape

• Cranial ridges

• Brow ridges

Lines of evidence that support the idea of common ancestors for living hominids including humans:

• Fossil evidence: Evidence from fossils of different ages show that the anatomical characteristics of organisms changed gradually over time.

• Emphasis on evolutionary trends provided by the anatomical features of fossils of the following three genera:

- Ardipithecus

- Australopithecus

- Homo as well as:

- The age of each fossil found/time-line for the existence of the three genera

- The fossil sites where they were found: emphasis on the fossil sites that form a part of the Cradle of Humankind

- The scientists who discovered them

• Genetic evidence: mitochondrial DNA

• Cultural evidence: tool-making Out of Africa

hypothesis

Evidence for the Out of Africa hypothesis:

• Fossil evidence: information on each of the following fossils that serve as

evidence for the Out of Africa hypothesis:

- Ardipithecus (fossils found in Africa only)

- Australopithecus (fossils found in Africa only, including Karabo, Littlefoot, Taung Child, Mrs Ples)

- Homo (fossils of Homo habilis found in Africa only; oldest fossils of Homo erectus found in Africa, while the younger fossils were found in other parts of the world)

• Genetic evidence: mitochondrial DNA

Timeline for the existence of different species of the genus Homo and the significant features of each type of fossil to illustrate the differences amongst them

Interpretation of phylogenetic trees proposed by different scientists showing possible evolutionary relationships as it applies to hominid evolution



The atmosphere and climate change

Sources of carbon dioxide emissions and methane emissions (greenhouse gases)

The greenhouse effect and its importance for life on Earth

Difference between the greenhouse effect and the enhanced greenhouse effect

Global warming: due to an increase in greenhouse gases (enhanced greenhouse effect)

Effects of global warming: desertification, drought and floods

Deforestation and its influence on the CO2 concentration in the atmosphere

Carbon footprint: ways of reducing our 'carbon footprint'

Causes and consequences of ozone depletion

Strategies to decrease ozone depletion Water availability

Influence of the following factors on the availability of water:

• Construction of dams

• Destruction of wetlands

• Exotic plantations and depletion of the water table

• Water wastage

• Cost of water

• Poor farming practices

• Droughts and floods

• Boreholes and its effects on aquifers Water quality

Factors that reduce water quality:

• Eutrophication and algal bloom

• Domestic, industrial and agricultural use – leading to pollution and disease

• Mining

• Alien plants, e.g. Eichornia

• Thermal pollution

Role of water purification in improving the quality of water

Role of the recycling of water in improving the quality of water

Food security Definition of food security

Factors that influence food security:

• Human exponential population growth

• Droughts and floods (climate change)

• Alien plants and the reduction of agricultural land

• The loss of wild varieties: impact on gene pools

• Wastage

• Genetically engineered foods

• Poor farming practices such as:

- Monoculture

- Overgrazing and the loss of topsoil

- The use of fertilisers

- The use of pesticides

Loss of biodiversity

The importance of maintaining biodiversity

Factors that reduce biodiversity:

• Habitat destruction through:

- Farming methods (overgrazing and monoculture)

- Golf estates

- Mining

- Urbanisation

- Deforestation

- Loss of wetlands and grasslands

• Poaching (e.g. rhino horn, ivory, 'bush meat')

• Alien plant invasions

Factors that reduce the loss of biodiversity:

• Control of alien plant invasion using mechanical, chemical and biological methods

• The sustainable use of the environment using any ONE of the following examples: devils' claw, rooibos, fynbos, the African potato (Hypoxis) or Hoodia Solid waste disposal

The need to reduce solid waste or find ways of managing it

Aspects of solid-waste disposal:

• Ways in which dumpsites can be managed for rehabilitation and prevention of soil and water pollution

• The use of methane from dumpsites for domestic use, such as heating and lighting

• The need for recycling

• The need for safe disposal of nuclear waste

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